Dhx9 atpase assay
WebOct 17, 2006 · Interacts (via DRBM, OB-fold and RGG regions) with NUP98 (via N-terminus); this interaction occurs in a RNA-dependent manner and stimulates DHX9-mediated ATPase activity and regulates transcription and splicing of a subset of genes (PubMed:28221134). WebSep 28, 2024 · DHX9 enhanced NF-κB-mediated transactivation in the nucleus, which required its ATPase-dependent helicase (ATPase/helicase) domain, but not the …
Dhx9 atpase assay
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WebCompare DHX9 Kits from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. WebJun 6, 2024 · The RNA helicase DHX9 associates with IGS-rRNA pRNA is a 250–300 nucleotide transcript that derives from processing of the 2-kb …
WebDHX9 has also been shown to directly interact with CBP to aid in recruitment of RNA polymerase II to CRE-containing genes, 46 with the ATPase activity of DHX9 being important for transcriptional induction of genes regulated by a CRE sequence. 47 These data prompted us to test if Nup98 could stimulate CBP-dependent transcription in a … WebFeb 21, 2024 · We have shown using a combination of in vitro and in vivo assays that Nup98 directly binds DHX9 in the nucleoplasm and this interaction is facilitated by RNA. Importantly, binding of Nup98 to …
WebRNA-seq analysis showed that the Dhx9 deficiency caused an upregulated DNA damage response pathway and Gadd45, Cdkn1a, Cdc25, Wee1, and Myt1 expression to induce … WebAug 1, 2012 · The goal of this project is to develop HTS assays that will allow discovery of small molecule inhibitors of DHX9, which in turn, can be used in cell and animal models to examine the consequences of inhibition of this protein on cancer cell survival. ... Pelletier, Jerry (2015) Establishment of a Primary Screening Assay for the DHX9 Helicase ...
WebAug 1, 2024 · The complex biology of DHX9 was recently reviewed [221]. Helicase and competition assays proved DHX9 to bind and resolve G4s with stronger kinetics toward rG4 and strict ATP-dependence in vitro, as confirmed by the complete loss of G4-unwinding function upon ATPase inhibition by N-ethylmaleimide poisoning [222]. northern edge investment bankWebImportantly, binding of Nup98 to DHX9 stimulates the ATPase activity of DHX9, and a transcriptional reporter assay suggests Nup98 supports DHX9-stimulated … northern edge milled flaxseedWebMar 21, 2024 · Entrez Gene Summary for DHX9 Gene. This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that … northern edge coffeeWebMay 8, 2024 · Because DHX9-depleted cells exhibit a termination defect (Figures 4 H and 4I), we examined the potential role of DHX9 in preventing R-loop-associated DNA damage. For these studies, we used the Top1 inhibitor camptothecin (CPT), which is known to promote R-loop accumulation and replication- and transcription-associated DNA damage … northern edge farmington nmWebMar 29, 2024 · d, Luciferase assays show that DHX9 depletion significantly reduces expression of inverted-repeat-Alu-containing 3′ UTR elements, which can be alleviated … northern edge wood products ltdWebKeywords: ATPase assay, ATA, aurintricarboxylic acid, DHX9, HTS, RNA binding, RNA helicase. Abstract: The RNA helicase DHX9 is an ATP-dependent DExH box helicase that can unwind DNA and RNA. Much evidence has implicated DHX9 at multiple levels of gene expression regulation ranging from genome stability and replication, to transcriptional ... how to roast a butternut squashWebJul 23, 2010 · The SAT motif has been shown by mutagenesis to uncouple ATPase activity from RNA helicase activity in both DEAD-box 46, 47, ... NTPase assay. DHX9 325–840 was diluted into an assay buffer [20 mM Hepes (pH 7.5), 3 mM MgCl 2, 300 mM NaCl, 10% glycerol, and 2 mM TCEP] up to final concentrations of 7–80 nM. The assay was … how to roast a canadian person