WebJun 27, 2011 · Measurement of PRRSV-specific antibody levels by IDEXX ELISA after vaccination according to the viral titer. For the experimental efficacy test according to the viral titer, PRRSV suspensions of titers of 10 4, 10 5, or 10 6 PFU/mL were inactivated with binary ethylenimine (BEI) and inoculated into pigs. From 21 to 56 days after the first … WebAug 1, 1990 · The preparation and application of the aziridine compound binary ethylenimine (BEI) and the necessary conditions for and controls of the inactivation …
Sucrose density gradient centrifugation and cross-flow ... - PubMed
WebInactivated virus vaccines of varying composition are available commercially. Typically, virus is used to infect a suspension or monolayer cell culture and the resulting preparation is clarified, inactivated with ethyleneimine and concentrated. The antigen is usually blended with oil or aqueous adjuvant for vaccine formulation. WebAbstract. Experiments were carried out to inactivate F.M.D. viruses with the use of binary ethylene-imine. It was found that inactivation was optimal when the agent was used at … router huawei b525
Virus inactivation and protein modifications by ethyleneimines
WebJan 14, 2004 · Results of the binary ethylenimine inactivation trial suggested that standardised periods of 5 or 8 hours would be suitable to ensure effective and complete inactivation for a number of different arboviral antigens. ... Tollis M, Menna F, Papparella V: A preliminary vaccine potency trial of a Newcastle disease virus inactivated with binary ... WebOct 1, 2001 · The inactivation is expressed in reduction factors (RF): RF = log 10 initial virus titre virus titre after inactivation. 2.5. Protein modifications Test proteins (myoglobin, ovalbumin, BSA; 150 μM) were incubated in the presence of ethyleneimine (20 mM) at different pH values (6–8) for 24 h at room temperature. Webtivating agents on Newcastle disease virus (NDV) and inuenza virus, the results indicated that the potencies of FA and BPL inactivated vaccines were dierent [11, 12]. In case of binary-ethylenimine (BEI), its inactivation mechanism is similar to BPL and reacts very little with viral proteins. However, some studies showed that BEI stray t shirt